![]() "The protonophore CCCP interferes with lysosomal degradation of autophagic cargo in yeast and mammalian cells". "The pharmacological regulation of cellular mitophagy". ^ a b c Georgakopoulos, Nikolaos D Wells, Geoff Campanella, Michelangelo (2017)."Membrane-Activated Fluorescent Probe for High-Fidelity Imaging of Mitochondrial Membrane Potential". ^ Lin, Bo Liu, Yunfan Zhang, Xiaoping Fan, Li Shu, Yang Wang, Jianhua (November 10, 2021).International Review of Cell and Molecular Biology. ^ Bárcena, Clea Mayoral, Pablo Quirós, Pedro M."Targeting metabolic pathways for extension of lifespan and healthspan across multiple species". Moskalev, Alexey Perrimon, Norbert (December 2020). elegans longevity pathways converge to decrease mitochondrial membrane potential". Behrendt, Maciej DeCorby, Adrienne Gášková, Dana (July 2009). The International Journal of Biochemistry & Cell Biology. "Factors and processes involved in membrane potential build-up in yeast: diS-C3(3) assay". Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. "Effect of carbonyl cyanide m-chlorophenylhydrazone (CCmCP) on the dimerization of lipoprotein lipase". Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP).Outside of its effects on mitochondria, CCCP may also disrupt lysosomal degradation during autophagy. In order to set up the best conditions for MP evaluation, the staining was. It is routinely used as an experimental uncoupling agent in cell and molecular biology, particularly in the study of mitophagy, where it was integral in discovering the role of the Parkinson's disease-associated ubiquitin ligase Parkin. The ionophore agent CCCP at different concentrations (0.5, 1, and 20 M) was used to validate the staining protocol. The chemical acts essentially as an ionophore and reduces the ability of ATP synthase to function optimally. CCCP causes an uncoupling of the proton gradient that is established during the normal activity of electron carriers in the electron transport chain. elegans models, suggesting a degree of hormesis. In general, CCCP causes the gradual destruction of living cells and death of the organism, although mild doses inducing partial decoupling have been shown to increase median and maximum lifespan in C. It is a nitrile, hydrazone and protonophore. Carbonyl cyanide m-chlorophenyl hydrazone ( CCCP also known as malononitrile) is a chemical inhibitor of oxidative phosphorylation.
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